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HPLC Course



Illustrating chromatography


When working with method development or trouble shooting of liquid chromatographic methods, one usually comes to a point where the method is not working as well as one would like it too. The action taken is often to try to solve a problem by testing more or less vague ideas. The reasoning, “Maybe the problem can be solved if I change this or that parameter?” is frequently applied in practice. It is tempting to believe that the only way to solve a problem is by trial and error or by applying some tricks obtained from a colleague or some other source.


One may ask; “Is this the most rational approach to problem solving in liquid chromatography?”.This question is important for those who work with the development of chromatographic methods.


For more than 40 years, thousands of scientists and engineers have worked on the development of HPLC. The result is one of the most versatile techniques that we have in chemical science today. Furthermore, the development is still going on with thousands of papers and many books being published every year. All this has been accomplished because there is an understanding of the basic physico-chemical principles of the chromatographic process. As an expert in liquid chromatography you will also need to understand these basic principles and to learn how they are implemented into your daily work.


Academy of Chromatography offers you courses in liquid chromatography at different theoretical levels. The purpose of all our courses is to give you an understanding of the basic underlying principles of liquid chromatography and to show their practical consequences. The courses are aimed at those who work with method development and troubleshooting in liquid chromatography. Read more about our courses.


You will find some tutorial material in the following pages on this site. The tutorials are divided into four different categories: "HPLC Novice", "HPLC user", "HPLC method developer" and "HPLC scientist". To test your level of knowledge, each category has a self test. The terminology of chromatography is found in a separate section.


What is chromatography? Click here to see an animated simulation of the chromatographic process!


Do you want to learn more about reversed phase chromatography? Visit


I wish you good luck with your studies in chromatography. Enjoy it, it´s interesting!


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