Are you working with advanced HPLC development?
Those who have high aspirations and want to become qualified experts or scientitsts in liquid chromatography now have a unique opportunity to apply to an Advanced Course in Liquid Chromatography. The course is given by Professor Jan Ståhlberg, Academy of Chromatography, and can be followed on-line. Read more about this course.
Below you find some basic material in chromatographic theory.
Development of advanced HPLC methods requires a thorough understanding of chromatographic theory. This site will give you a start.
We recommend you to first go to the animation in the "HPLC developer" section. Look at the three animations and try to understand the reason for their different outcomes. This is a good start for further studies.
Secondly, a good understanding of how the basic mass balance equation is derived is well spent time.
From a scientific point of view chromatography comprises several disciplines. Important areas that a chromatographer at this level must be acquainted with include:
- Intermolecular forces.
- Theories of solutions
- Surface chemistry
- Mass transfer
Furthermore, a thorough understanding of the implications of the mass balance equation for chromatography is needed. It is also important to have a good understanding of the theory for multicomponent chromatography .
In conclusion, a person working at this level needs a good background in physical sciences as well as a technical knowledge about the equipment.
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